In this study, we developed a novel, multiplex qPCR assay for simultaneous detection of RIG-1, MDA5, and IFIT-1 at the mRNA level.The assay was validated in A549 cells transfected with in vitro transcribed RNAs.Both exogenous RNA-GFP and self-amplifying (saRNA-GFP) induced significant expression of RIG-1, MDA5, IFIT-1, as well as type I and III int
PEGylation technology, that is grafting of poly(ethylene glycol)(PEG) to biologics, vaccines and Bun Pan Carriers nanopharmaceuticals, has become a cornerstone of modern medicines with over thirty products used in the clinic.PEGylation of therapeutic proteins, nucleic acids and nanopharmaceuticals improves their stability, pharmacokinetic and biodi
Effective antibiotic therapy requires reliable data on the dynamics of sensitivity changes in each region.The aim of the study was to determine the sensitivity to antibiotics and antilysozyme activity of Staphylococcus aureus strains isolated from the blood of patients with sepsis over the period from 2017 to 2019.An strapless-strapon increase in t
Abstract Many widely used reservoir simulators include an EOR option, but there is no feature available in a commercial simulator in the oil industry that simulates microbial enhanced oil recovery LD (MEOR) process.Eclipse, one of the popular reservoir simulators, does not have this option.In this study the Eclipse reservoir simulator has been modi